US EPA

1053092 

Journal Article 

Acute bromadiolone intoxication 

Grobosch, T; Angelow, B; Schönberg, L; Lampe, D 

2006 

Yes 

Journal of Analytical Toxicology
ISSN: 0146-4760
EISSN: 1945-2403 

United States 

30 

4 

281-286 

English 

16803669 

A 55-year-old man came to the hospital with a bleeding wound on his tongue. The coating of his tongue was green, and his sputum was red. Because an increased international normalized ratio-value was measured, a blood sample was sent to our laboratory with the suspicion of coumarin intoxication. Liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analysis confirmed the poisoning was by bromadiolone, with its maximum serum concentration at 440 microg/L. The analysis of further samples resulted in a calculated elimination half-life of 140 h. The analytical method described was developed for the determination and quantitation of bromadialone using LC-MS. This method is suitable for the simultaneous identification and quantitation of 10 indirect anticoagulants in human serum, which include five superwarfarins (brodifacoum, bromadiolone, difenacoum, difethialone, and flocoumafen) as rodenticides licenced in Germany and five other vitamin K antagonists (acenocoumarol, coumatetralyl, coumachlor, phenprocoumon, and warfarin). The method is based on an acidic (pH 4.2) liquid-liquid extraction followed by LC-ESI-MS analysis. Analytical separation was carried out using an Atlantis C18 column (2.1 x 20 mm, 3 microm). The mobile phase consisted of methanol/0.1% formic acid; the flow rate was 0.6 mL/min, and the time needed for analysis was 5 min. The lower limit of quantitation was 5 microg/L (signal-to-noise > 10). 

pH effects; Antagonists; Rodenticides; Sputum; Bleeding; Anticoagulants; Warfarin; Wounds; Poisoning; Coumarin; Methanol; Intoxication; Formic acid; Case reports; Vitamin K; Quantitation; Tongue; Spectrometry; Hospitals; Coatings