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Citation
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HERO ID
1055437
Reference Type
Journal Article
Title
Nigella sativa L. seed extract modulates the neurotransmitter amino acids release in cultured neurons in vitro
Author(s)
El-Naggar, T; Gómez-Serranillos, MP; Palomino, OM; Arce, C; Carretero, ME
Year
2010
Is Peer Reviewed?
Yes
Journal
Journal of Biomedicine and Biotechnology
ISSN:
1110-7243
EISSN:
1110-7251
Volume
2010
Page Numbers
398312
Language
English
PMID
20625485
DOI
10.1155/2010/398312
Abstract
Nigella sativa L. (NS) has been used for medicinal purposes since ancient times. This study aimed to investigate the cytotoxicity of NS dry methanolic extract on cultured cortical neurons and its influence on neurotransmitter release, as well as the presence of excitatory (glutamate and aspartate) and inhibitory amino acids (gamma-aminobutyric acid-GABA-and glycine) in NS extract. Cultured rat cortical neurons were exposed to different times and concentrations of NS dry methanolic extract and cell viability was then determined by a quantitative colorimetric method. NS did not induce any toxicity. The secretion of different amino acids was studied in primary cultured cortical neurons by high-performance liquid chromatography (HPLC) using a derivation before injection with dansyl chloride. NS modulated amino acid release in cultured neurons; GABA was significantly increased whereas secretion of glutamate, aspartate, and glycine were decreased. The in vitro findings support the hypothesis that the sedative and depressive effects of NS observed in vivo could be based on changes of inhibitory/excitatory amino acids levels.
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IRIS
•
Methanol (Non-Cancer)
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PubMed
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