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1107788 
Journal Article 
Antioxidant activity and rosmarinic acid changes in salicylic acid-treated Thymus membranaceus shoots 
Perez-Tortosa, V; Lopez-Orenes, A; Martinez-Perez, A; Ferrer, MA; Calderon, AA 
2012 
Yes 
Food Chemistry
ISSN: 0308-8146
EISSN: 1873-7072 
ELSEVIER SCI LTD 
OXFORD 
130 
2 
362-369 
English 
WOS:000296267400021 
In order to characterise the antioxidant activity of Thymus membranaceus Boiss. subsp. membranaceus, an endemic species in Southeast Spain, five different analytical methods were used. Water, methanol and hexane extracts obtained from 60-day-old in vitro-grown shoots were assayed for their antioxidative properties using DPPH (1,1-diphenyl-2-picrylhydrazyl radical), ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonate), FRAP (ferric reducing antioxidant power), reducing power and conjugated diene assays. Total soluble phenol content, as well as rosmarinic acid, in the different extracts were also determined. Methanolic extracts exhibited the best antioxidant activity and the highest amounts of total soluble phenolics. A single application of salicylic acid (10 μM) on culture media resulted in an increase in rosmarinic acid and phenolic levels, which in turn improved the extracts' antioxidant properties. These current findings open new opportunities for obtaining valuable natural antioxidants for commercial exploitation by using tissue culture systems. © 2011 Elsevier Ltd. All rights reserved. 
Thymus membranaceus; Antioxidant activity; Total phenolic content; Rosmarinic acid; Shoot cultures 
IRIS
• Methanol (Non-Cancer)
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