US EPA

1116564 

Journal Article 

Antimicrobial Activity Evaluation of Cassia spectabilis Leaf Extracts 

Krishnan, N; Ramanathan, S; Sasidharan, S; Murugaiyah, V; Mansor, SM 

2010 

Yes 

International Journal of Pharmacology
ISSN: 1811-7775 

Asian Network for Scientific Information 

FAISALABAD 

6 

4 

510-514 

English 

10.3923/ijp.2010.510.514 

WOS:000285675900028 

The aim of the present study was to characterize the antimicrobial properties of various crude extracts of the Cassia spectabilis leaf against bacteria and yeast. Acetone, n-hexane, dichloromethane, ethy acetate and methanol extracts of C. spectabilis leaves were evaluated in vitro against Gram positive bacterii (Bacillus subtilis and Staphylococcus aureus), Gram negative bacteria (Escherichia coli, Salmonella typhi anc Pseudomonas aeroginosa) and yeast (Candida albicans). The Minimum Inhibitory Concentration (MIC) Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentration (MFC) were determinec using micro dilution assay. Methanol showed the highest yield (14.12%) followed by dichlorometham (8.37%), acetone (6.66%), ethyl acetate (4.76%) and n-hexane (1.80%). Acetone and methanol crude extracts showed a good antimicrobial activity with MIC values ranging from 0.625 to 2.5 mg mL super(-1) and MBC or MFC values ranging from 1.25 to 5 mg mL super(-1). The MIC, MFC and MBC values of these extracts were 10 to 80 times less potent than standard antimicrobial drugs, amoxilin and miconazole nitrate. 

Bacteria; yeast; minimum inhibitory concentration; crude extracts