US EPA

1116695 

Journal Article 

Protective Effects of Green tea (Camelia sinensis), Hypericum perforatum and Urtica dioica on Hepatic Injury and Lymphocyte DNA Damage Induced by Carbon Tetrachloride in Wistar Rats 

Bitiren, M; Musa, D; Ozgonul, A; Ozaslan, M; Kocyigit, A; Sogut, O; Guldur, ME; Kilic, IH; Karakilcik, AZ; Zerin, M 

2010 

Yes 

International Journal of Pharmacology
ISSN: 1811-7775 

6 

3 

241-248 

WOS:000285675700010 

The present study was planned to investigate the protective effects of herbal methanol extracts of Hypericum perforatum (HP) and Urtica dioica (UD) and herbal agueous extract of Camelia sinensis (CS)-containing antioxidants on carbon tetrachloride (CCl sub(4))-induced hepatic injury and lymphocyte DNA damage. Adult male albino rats (n 50) were separated into five groups of ten each, as follows: control group; CCl sub(4), group; CCl sub(4)+HP extract group; CCl sub(4)+UD extract group; CCl sub(4)+CS extract group. All extract groups were fed with 200 mg kg super(-1) extracts of HP, CS and UD, respectively once every alternate day for 7 weeks. CCl sub(4) injections were applied to the CCl sub(4) and extract groups at the dose of 0.4 mL kg super(-1). Malondialdehyde, total oxidant status and total antioxidative capacity levels and catalase, superoxide dismutase and glutathione peroxidase activities were significantly changed in the CCl sub(4) group and indicated increased oxidative stress. The DNA damages detected via commet assay were significantly higher than in the CCl sub(4) group. Treatment by extracts of HP, CS or UD were significantly decreased this oxidative stress and ameliorated lymphocyte DNA damage. These results indicate that the herbal extracts of HP, UC and CS have beneficial effects on liver and lymphocyte DNA damage induced by CCl sub(4) probably due to their antioxidant properties. 

Herbal extracts; oxidative stress; DNA damage; liver; carbon tetrachloride