US EPA

1225338 

Journal Article 

Antibacterial activity and characterization of secondary metabolites isolated from mangrove plant Avicennia officinalis 

Valentin, BB; Meenupriya, J; Joel, EL; Naveena, DE; Kumar, S; Thangaraj, M 

2010 

Yes 

Asian Pacific Journal of Tropical Medicine
ISSN: 1995-7645 

3 

7 (Jul 2010) 

412-420 

Objective: To explore antibacterial activity and characterization of secondary metabolites isolated from mangrove plant Avicennia officinalis (A. officinalis). Methods: In the present study the leaf extracts of A. officinalis were examined for its antibacterial potential using five different solvents against some reference strains of human pathogenic bacteria for the crude extract. Maximum activity was observed for ethyl acetate and hence different concentrations like 15 k L, 25 k L, and 50 k L of ethyl extracts was checked for its antibacterial activity. Partial purification of crude extract was carried by column chromatography and fractions were analyzed using gas chromatography-mass spectrometry (GC-MS) to identify compounds. Results: The crude ethyl acetate extracts of A. officinalis showed remarkable antibacterial activity with zones of inhibition of 13 mm against Eschericia coli (E. coli) and 11 mm against Staphylococcus aureus (S. aureus). Fraction 13 (ethyl acetate: methanol= 8: 2) as the most potent one against with the minimal inhibitory concentration of 30 mm against E. coli and 25 mm against S. aureus. The GC-MS results of active column fraction (F13) revealed that the active principals were a mixture of hydroxy- 4 methoxybenzoic acid, diethyl phthalate, oleic acid. Conclusions: The leaf extracts with proven antibacterial effects can clearly be directed towards cancer treatment as to inhibiting cancer cell growth. The limited number of test organisms owes to a constraint of resource. So, the effect of strong bursts of leaf extracts on human pathogenic bacteria should further be tested on a wide range of test organisms. 

Antibacterial activity; Gas chromatography; Cancer; Column chromatography; Mangroves; Leaves; Oleic acid; Avicennia officinalis; Purification; Solvents; diethyl phthalate; Staphylococcus aureus; Escherichia coli; Mass spectroscopy; Ethyl acetate; Secondary metabolites