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1225472 
Journal Article 
Toxicity Study of Malaysian Rubber (Hevea brasiliensis) Seed Oil as Rats and Shrimps Tests 
Abdullah, BM; Salimon, J 
2010 
Yes 
Asian Journal of Biochemistry
ISSN: 1815-9923
EISSN: 1815-9931 
5 
1 (Jan 2010) 
33-39 
English 
The lipid fraction of Malaysian rubber seed was extracted and analyzed for toxicological compounds such as linamarin. Analytical methods (rat's toxicological test and brine shrimps test) were applied to determine the presence of such compounds. Malaysian rubber seed oil was extracted using different solvents (hexane and chloroform+methanol). The oil was found not to contain unusual fatty acids and it is rich in essential unsaturated fatty acids, particularly linoleic (37.28 plus or minus 0.10%) and linolenic (19.22 plus or minus 0.21%). Malaysian rubber seed oil showed no toxic potential toward the rats. Bioassay experiments, in which shrimps were used as test organisms, were performed to evaluate the toxicity of linamarin extracted from the oil. The median lethal concentration (LC sub(50)) was found to be 211.70%. The results showed no acute toxicity effects, probably since Malaysian rubber seed oil was not found to contain any hazardous linamarin. 
n-Hexane; Oil; Fatty acids; Rubber; Solvents; Seeds; Lipids; Brines; Acute toxicity; Hevea brasiliensis 
IRIS
• Methanol (Non-Cancer)
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