US EPA

1227263 

Journal Article 

"In vitro Antibacterial Potential of Alkaloids of Samanea saman (Jacq.) Merr. Against Xanthomonas and Human Pathogenic Bacteria" 

Raghavendra, MP; Satish, S; Raveesha, KA 

2008 

Chk 

World Journal of Agricultural Sciences
ISSN: 1817-3047 

4 

1 

100-105 

Aqueous extract, different solvent extracts and isolated constituents (alkaloids) of leaves of Samanea saman (Jacq.) Merr. (fabaceae) were assayed for antibacterial activity by cup diffusion method against three phytopathogenic Xanthomonas pathovars viz., Xanthomonas axonopodis pv. malvacearum, X. a. pv. phaseoli and X. campestris pv. vesicatoria and 14 human pathogenic bacteria. The pathovars are associated with angular leaf spot of cotton, common blight of bean and bacterial spot of tomato respectively. Aqueous and methanol extracts showed significant antibacterial activity against all pathovars of Xanthomonas and the activity varied among 14 human pathogenic bacteria. Methanol extract was subsequently fractioned and monitored by bioassay leading to the isolation of active fraction by further phytochemical analysis. This active fraction recorded highly significant antibacterial activity in vitro (MIC 6, 6 and 4 mu g ml super(-1) for Xanthomonas pathovars and. 3-11 mu g ml super(-1) for human pathogenic bacteria) compared with synthetic antibiotics such as Bacterimycin 2000 and Sreptocycline tested at recommended dosage for phytopathogenic bacteria and Gentamycin and Streptomycin discs for human pathogenic bacteria. The active fraction was further confirmed as alkaloids. 

Antibacterial activity; Leafspot; Fabaceae; Xanthomonas axonopodis; Leaves; Antibiotics; Diffusion; Blight; Methanol; Beans; Xanthomonas; Alkaloids; Minimum inhibitory concentration; Lycopersicon esculentum; Streptomycin; Solvents; Cotton