US EPA

1230473 

Journal Article 

Determination of Isoproturon and Its Metabolite 4-Isopropylaniline in Soil by High Performance Liquid Chromatography 

Zhang, J; Li, Fshi 

2005 

1 

Nongye Huanjing Kexue Xuebao / Journal of Agro-Environment Science
ISSN: 1672-2043 

24 

5 (Oct 20 

1000-1002 

Isoproturon [34123-59-6] is a selective herbicide widely used for agricultural purpose. Its metabolites should be considered in studies on the migration of isoproturon and in the eco-toxicological evaluation. A high performance liquid chromatography (HPLC) procedure is developed for the simultaneous determination of isoproturon and its main metabolite, 4-isopropylaniline, in soil. The stationary phase used was Eclipse XDB-C sub(18) (4.6 mmx150 mm, 5mm) packed in a stainless steel column. The UV detector operated at 245 nm. The flow rate was 0.3 mL times min super(-1). A 70:30 (V/V) methanol-water solution was used for the chromatography elution to separate. Overlapping of isoproturon and 4-isopropylaniline peaks in the selected conditions was resolved by changing the initial mobile phase to 70:30 (V/V) acetonitrile-water solution. The method allowed analyses at room temperature with a total run time less than 10 min per sample. For isoproturon and 4-isopropylaniline, the linear range of working curve were 25-200 mg times L super(-1), linear correlation 0.9946 and 0.9976, the detection limits 0.042 mg times L super(-1) and 0.45 mg times L super(-1), the relative standard deviations 0.66% and 0.67%, respectively. Isoproturon and 4-isopropylaniline were ultrasonically extracted with acetonitrile from soil samples. The average recovery rates of standard additions were 91.76% and 92.57%, respectively. These suggested the method was simple and accurate, suitable for routine residue analyses and applicable to control isoproturon production in industry. 

Herbicides; Sampling methods; Ultrasonics; Temperature; Soil; Separation processes; Metabolites; Liquid chromatography; Chemical analysis; 2005)