US EPA

1358642 

Journal Article 

Speciation of arsenic and selenium via anion-exchange high performance liquid chromatography with sequential plasma emission detection 

Mccarthy, JP; Caruso, JA; Fricke, FL 

1983 

Yes 

Journal of Chromatographic Science
ISSN: 0021-9665 

HEEP/84/04927 

21 

9 

389-397 

eng 

HEEP COPYRIGHT: BIOL ABS. The speciation and quantification of arsenate, arsenite, selenate and selenite was achieved by anion-exchange HPLC (high performance liquid chromatography) with sequential inductively coupled plasma emission (ICP) detection. Earlier work demonstrated the applicability of the ICP for multi-element HPLC detection of metal-containing moieties by using a polychromator system. Sequential detection for this purpose offered more flexibility than the fixed wavelength polychromator detector, yet was highly applicable to systems in which analyte signal was presented sequentially to the detector. Since the spectrometer for sequential ICP detection was less complex than its polychromator counterpart, the instrumentation expense was reduced. This approach was more demanding on the chromatography since it monitored only 1 channel at a time. It extracted multi-element speciation information from a single run. Baseline resolution was not necessary with sequential detection if quantification was carried out using the peak height. Detection limits were 1.8 ng As/s and 3.9 ng Se/s. Absolute detecting limits were 52 ng As(III), 140 ng Se(IV), 57 ng As(V) and 91 ng Se(VI). Reproducibility at the 2.8 mug level was 4, 7, 6 and 6%, respectively, for As(III), SE(IV), As(V) and Se(VI), with a linear response through 9 mug for the lower oxidation states and 5 mug for the higher oxidation states.