US EPA

1372528 

Technical Report 

Relationship of Metallothionein Induction to the Heat Shock Response 

Yang, JM; Bowman, PD; Deaton, MA; Schuschereba, ST; Stuck, BE 

1993 

NTIS/02980137_2 

GRA and I 

GRA and I 

Heat shock proteins (hsps) and Metallothioneins (MTs) are sets of proteins specifically synthesized by cells in response to sublethal injury such as heat, heavy metals or radiations. This dissertation investigated the relationship of metallothionein induction to the heat shock response. Sodium arsenite, which induces both hsps and MTs was used to establish a model system for investigation. Electrophoresis conditions that allowed simultaneous identification and quantitation of hsps and MTs were then developed. Identification of MTs was achieved by (35S) cysteine and methionine labeling and (109Cd)-binding. The results of this study indicate that MTs undergo increased synthesis following both heat shock and sodium arsenite treatment. The level of increase of MTs after heat shock was about one-tenth of the increase after sodium arsenite treatment. These results suggest that MTs are heat shock and stress proteins....Sodium arsenite, Heat shock proteins, Stress points. 

Cells; Cysteine; Electrophoresis; Heavy metals; Identification; Methionine; Models; Response; Sodium; Synthesis; Theses; Wounds and injuries; Radiation; Sulfur; Cadmium; Stresses; Peptides; Sublethal dosage; Metabolism; Metallothionein induction; *HSPS(Heat Shock Proteins); Arsenites; Biosynthesis