US EPA

1445585 

Journal Article 

A novel method for quantification of ethanol and methanol in distilled alcoholic beverages using Raman spectroscopy 

Boyaci, IH; Genis, HEfe; Guven, B; Tamer, U; Alper, N 

2012 

Yes 

Journal of Raman Spectroscopy
ISSN: 0377-0486 

43 

8 

1171-1176 

10.1002/jrs.3159 

WOS:000307550100031 

In this study, direct quantification of ethanol and
methanol in distilled alcoholic beverages using Raman spectroscopy was performed. Raman spectra
of varying ethanolmethanol mixtures were obtained, baseline corrections were made, and the data
were normalized using Raman scattering intensity of an internal standard (acetonitrile, 921?cm1).
Then, calibration graphs were produced for ethanol and methanol concentrations in the ranges of
07?M and 010?M, respectively. Accurate R2 values of the calibration graphs proved the notable
linear correlations (0.998 for ethanol and 0.998 for methanol). The method was validated based on
linearity, sensitivity, intraday and interday repeatability, and recovery tests. The limit of
detection and limit of quantification values of the validated method were determined for ethanol
concentration as 1.2 and 3.7?mM, and for methanol concentration as 3.4 and 10.3?mM, respectively.
The ability of the developed method to detect ethanol and methanol concentrations in real samples
was also investigated. The results of the developed method were compared with the experimental
results from traditional method and high correlation value (R2?=?0.926) was obtained. Besides
being sensitive and cheap, the developed method is rapid with the analysis time of less than 30
s. Furthermore, it eliminates labor-consuming operations, chromatographic separation, and
measurement error due to the high number of experiment steps in the standard method. Copyright
(c) 2012 John Wiley & Sons, Ltd. 

ethanol; methanol; alcoholic beverages; Raman spectroscopy