Determination of Folic Acid and its Related Substances of the Ferrous Fumarate and Folic Acid Dispersible Tablet by HPLC | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

1445968

Reference Type

Journal Article

Title

Determination of Folic Acid and its Related Substances of the Ferrous Fumarate and Folic Acid Dispersible Tablet by HPLC

Author(s)

Chen, Q; Liu, YL; Luo, YH; Luo, M; Luo, LY

Year

2012

Volume

Issue

Page Numbers

555-560

Web of Science Id

WOS:000308119000010

Abstract

The aim of the present study was to establish a method for
the determination of folic acid and related substances content in dispersible tablets. Method: A
Shim-pack VP-ODS C-18 reversed phase column (4.6 mm X 250 mm) was used. The mobile phase
consisted of methanol-phosphate buffer (20:80) with a pH of 6.3. The flow rate was 1.0 mL/min,
and the detection wavelength was 277nm and the column temperature was 30 degrees C. Results: The
calibration curve was linear in the range of 5 similar to 150 mu g/mL (r = 0.9998) for folic
acid. The minimal detection limit was 99.08 %, n = 9 and the related substances were well
separated. This method resulted to be convenient, accurate, selective and reliable, and can be
applied for the quality control of folic acid.

Keywords

Content determination; Folic acid and related substances; HPLC