US EPA

1446901 

Journal Article 

Cassia surattensis: An Indispensable Source for Natural Antioxidants 

Fatima, A; Aziz-ur-Rehman; Riaz, T; Abbas, N; Abbasi, MA; Siddiqui, SZ; Khan, Z 

2012 

Yes 

Asian Journal of Chemistry
ISSN: 0970-7077
EISSN: 0975-427X 

ASIAN JOURNAL OF CHEMISTRY 

SAHIBABAD 

24 

10 

4340-4344 

English 

WOS:000310771000022 

In this study, phytochemical screening and antioxidant
capacity of aqueous and organic fractions of aerial parts of Cassia surattensis L. was
investigated. Phytochemical tests showed that many secondary metabolites including flavonides,
steroids, alkaloids, saponins, tannins and anthraquinones etc., were present in the different
plant extracts like crude methanolic extract, n-hexane, chloroform, ethyl acetate, n-butanol
soluble fractions and remaining water fraction. First of all the antioxidant components were
extracted in methanol and then partitioning was done in solvents of different polarity.
Evaluation of the antioxidant activity and radical scavenging activity of these fractions was
done by using different antioxidant assays such as total phenolic contents (TPC), 2;2-diphenyl-1
-picrylhydrazil (DPPH) scavenging and the trolox equivalent antioxidant capacity (TEAC) of all
the studied fractions was evaluated by FRAP and ABTS (radical cation scavenging activity). All
the fractions exhibited significant antioxidant potential. The results showed that highest %
inhibition of DPPH was shown by ethyl acetate soluble fraction with IC50 4.00 +/- 1.7 mu g/mL of
butylated hydroxytoluene (BHT) equivalents. This fraction also contained highest total phenolic
contents (348.83 +/- 1.5 mg of gallic acid equivalents) and highest TEAC value (0.99 +/- 0.9)
measured by ABTS assay and methanolic crude extract exhibited highest total TEAC value (158.5 +/-
1.2) by FRAP method. 

Cassia surattensis L.; Phytochemical screening; DPPH; Total phenolic; Organic fractions