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1447609 
Journal Article 
Automated and standard extraction of antioxidant phenolic compounds of Hyssopus officinalis L. ssp angustifolius 
Hatipoglu, G; Sokmen, M; Bektas, E; Daferera, D; Sokmen, A; Demir, E; Sahin, H 
2013 
Yes 
Industrial Crops and Products
ISSN: 0926-6690 
ELSEVIER SCIENCE BV 
AMSTERDAM 
43 
1 
427-433 
English 
WOS:000311865000065 
This study was designed to determine the antioxidant
potentials of various extracts and the essential oil obtained from the leaves of Hyssopus
officinalis L. ssp. angustifolius (Lamiaceae). The hexane, chloroform and water extracts were
prepared by using an automated extraction system while essential oil was obtained from water-
distilation by using Clevenger-type apparatus. Methanol-water (1:1) extract was also prepared by
maceration. All extracts were evaluated for their abilities to scavenge free radicals (DPPH
method) and inhibition of the oxidation of linoleic acid (beta-caroten/linoleic acid) in vitro
test systems. In DPPH test, water extract exhibited higher radical scavenging activity. The 50%
inhibition (IC50) values of the water, methanol-water and chloroform extracts were 18.80, 28.80
and 250 mu g ml(-1), respectively. On the other hand, the non-polar extracts were active in
beta-carotene/linoleic acid test system. The essential oil did not exert activity in both tests.
Both total phenolics and total flavonoid contents were highest in the water extract possessing
4.70% (as gallic acid equivalent) and 1.30%, respectively. Proantho-cyanidine was confirmed to be
present at high concentrations in all samples, particularly in chloroform extracts (10,250 mg/L).
HPLC analysis of methanol extracts showed the presence of antioxidant phenolic compounds such as
chlorogenic acid (166.21 gg(-1)) and caffeic acid (111.09 gg(-1)), both were the major phenolics.
Automated extraction system provided better separation of the active principles than conventional
standard extraction procedures. (C) 2012 Elsevier B.V. All rights reserved. 
Hyssopus officinalis L. ssp angustifolius; Antioxidant activity; Plant phenolics 
IRIS
• Methanol (Non-Cancer)
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