US EPA

1448490 

Journal Article 

Formulation and In-vitro Evaluation for Sun Protection Factor of Crinum asiaticum Linn flower (Family-Amaryllidaceae) Extract Sunscreen Creams 

Kale, S; Kavade, E; Yadav, AV 

2012 

46 

2 

112-119 

WOS:000309649300004 

Crinum asiaticum Linn. flowers commonly known as white lily
flowers were explored to find flavonoids, so, thin layer chromatography was performed and some of
the flavonoids were identified as flavon (Apigenine), flavonol (Quercetin), flavan-3-ols
(Catechin), isoflavones (Daidzein), flavonol (Fisetin, Myricetin and Kaempferol). The reported
flavonoids were known to have sunscreen activity and hence SPF factor of the dried flower extract
(aqueous) was incorporated in topical formulation and evaluated in vitro with the help of
Optometric Model-290 SPF. Extraction of flowers was done in two steps; maceration in MeOH: Water
(9:1) followed by maceration in MeOH: Water (1:1) respectively. The two extracts were combined
and methanol was then evaporated with the help of rotary evaporator, the resultant aqueous
extract was used for Thin Layer Chromatography and then into topical formulation. In vitro
determination of SPF was done with the help of method specified by The Comite de Liaison de la
Parfumerie in Europe (COLIPA). The SPF was found to be 1.17 and Boots Star rating as 3,
indicating that Crinum asiaticum Linn flower extract (aqueous) can be considered as good
candidate for Sunscreen or as an additive in any other sunscreen formulation. 

Sun Protection Factor; in vitro method; flavonoids; Crinum asiaticum L. (Amaryllidaceae) flowers