US EPA

1450328 

Journal Article 

Development of a stability-indicating LC method for determination of a synthetic chalcone derivative in a nanoemulsion dosage form and identification of the main photodegradation product by LC-MS 

Mattos, CB; Deponti, VB; Barreto, F; Simoes, CMO; Andrighetti-Frohner, CR; Nunes, RJ; Steindel, M; Teixeira, HF; Koester, LS 

2012 

Yes 

Journal of Pharmaceutical and Biomedical Analysis
ISSN: 0731-7085
EISSN: 1873-264X 

70 

652-656 

English 

22884784 

10.1016/j.jpba.2012.07.021 

WOS:000309314600091 

This study aimed to develop and validate a stability
indicating LC for the determination of 5-(2-benzoylethenyl)-N-benzyl-2 methoxybenzenesulfonamide
(SCD - a synthetic chalcone derivative), and evaluate the degradation profile of the compound
under different stress conditions recommended by International Conference on Harmonization (ICH).
Chromatographic separation was performed on a silica C18 column, methanol-water mobile phase
70:30 (v:v), pH 5.0 adjusted with the addition of trifluoroacetic acid (TFA) at a flow rate of
1.0 mL min(-1); detection by UV absorption at 330 nm. The method was validated for linearity,
precision, accuracy, robustness and specificity. The SCD was subjected to forced degradation and
peak photodegradation product was well separated from the pure substance, with retention times
significantly different, indicating the specificity of the method. Second-order degradation
kinetics of SCD was observed under photodegradation and base-induced degradation. In order to
identify the product formed under photodegradation, liquid chromatography-tandem mass
spectrometry (LC-MS/MS) was performed. These results indicate that the method can be successfully
used in order to assay SCD in a nanoemulsion dosage form, and that this formulation has a
protective effect over SCD degradation. (C) 2012 Elsevier B.V. All rights reserved. 

Chalcone; Nanoemulsion; LC method; Kinetics study; LC-MS/MS