US EPA

1451214 

Journal Article 

SIMULTANEOUS SEPARATION AND QUANTIFICATION OF TARGETED GROUP OF COMPOUNDS IN PSORALEA CORYLIFOLIA L. USING HPLC-PDA-MS-MS 

Pandey, A; Niranjan, A; Misra, P; Lehri, A; Tewari, SK; Trivedi, PK 

2012 

Yes 

Journal of Liquid Chromatography and Related Technologies
ISSN: 1082-6076
EISSN: 1520-572X 

35 

18 

2567-2583 

10.1080/10826076.2011.636854 

WOS:000310738000006 

A rapid, sensitive, and simple high performance liquid chromatography photo diode array mass spectrometry (HPLC-PDA-MS-MS) method was developed for the simultaneous determination of nine compounds, namely, psoralen (furocoumarin), daidzein, genistein (isoflavonoids), daidzin and genestin (isoflavone glycosides), biochanin A (methoxylated isoflavone), and quercetin, kaempferol, and rutin (flavonols). Chromatographic separation of these nine molecules was performed on RP C18 column within 20 min. Elution was accomplished by the application of trifluoroacetic acid (0.05%) in water and methanol in gradient system with flow rate of 1.0 mL/min. PDA and mass spectrometry was employed for qualitative and quantitative analysis. The method is economical in terms of the time expended and the amount of solvent used for each analysis. The calibration curves were linear and ranged between 0.994 to 0.999; limit of detection (LOD) and quantification (LOQ) ranged between 1.02 to 1.18 and 2.23 to 3.34 and recovery ranged between 91.76% to 97.41%. The validated method was successively used to separate and quantitative measurement of these compounds in plant parts of Psoralea corylifolia. The developed method may be used in the quality control and standardization of plant extracts as well as herbal drugs and formulations. Supplemental materials are available for this article. Go to the publisher's online edition of Journal of Liquid Chromatography & Related Technologies to view the free supplemental file. 

furocoumarin; HPLC-PDA-MS-MS; method validation; polyphenols; Psoralea corylifolia; quantitative estimation