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Journal Article 
Determination of polyphenol components of Lonicera japonica Thunb. using liquid chromatography-tandem mass spectrometry: Contribution to the overall antioxidant activity 
Seo, OnN; Kim, GonSup; Park, S; Lee, JHan; Kim, YunHi; Lee, WonSup; Lee, SooJ; Kim, ChiY; Jin, JS; Choi, SK; Shin, SC 
Food Chemistry
ISSN: 0308-8146
EISSN: 1873-7072 
Three different mixtures of polyphenol components were
isolated from the leaf, flower and stem of Lonicera japonica Thunb. via aqueous 70% methanol
extraction followed by column chromatography over silica gel. The polyphenolic components of
these mixtures were analysed via high-performance liquid chromatography coupled with tandem mass
spectrometry (HPLC-MS/MS) and compared with the reported data. Among the 25 characterized
components, seven hydroxycinnamic acid derivatives and four flavonoid constituents were
identified for the first time. The polyphenol compounds were validated and quantified on the
basis of a representative polyphenol standard of the same group. The antioxidant activity of the
polyphenol mixtures of the three tissues was determined. The antioxidant activity of the leaf was
higher than that of the flower and stem. (C) 2012 Elsevier Ltd. All rights reserved. 
Polyphenols; Flavonoids; Lonicera japonica Thunb.; HPLC-MS/MS; Antioxidant activity; Metabolomes; Hydroxycinnamic acid 
• Methanol (Non-Cancer)
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