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Citation
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HERO ID
1451946
Reference Type
Journal Article
Title
Determination of polyphenol components of Lonicera japonica Thunb. using liquid chromatography-tandem mass spectrometry: Contribution to the overall antioxidant activity
Author(s)
Seo, OnN; Kim, GonSup; Park, S; Lee, JHan; Kim, YunHi; Lee, WonSup; Lee, SooJ; Kim, ChiY; Jin, JS; Choi, SK; Shin, SC
Year
2012
Is Peer Reviewed?
Yes
Journal
Food Chemistry
ISSN:
0308-8146
EISSN:
1873-7072
Volume
134
Issue
1
Page Numbers
572-577
DOI
10.1016/j.foodchem.2012.02.124
Web of Science Id
WOS:000304291400082
Abstract
Three different mixtures of polyphenol components were
isolated from the leaf, flower and stem of Lonicera japonica Thunb. via aqueous 70% methanol
extraction followed by column chromatography over silica gel. The polyphenolic components of
these mixtures were analysed via high-performance liquid chromatography coupled with tandem mass
spectrometry (HPLC-MS/MS) and compared with the reported data. Among the 25 characterized
components, seven hydroxycinnamic acid derivatives and four flavonoid constituents were
identified for the first time. The polyphenol compounds were validated and quantified on the
basis of a representative polyphenol standard of the same group. The antioxidant activity of the
polyphenol mixtures of the three tissues was determined. The antioxidant activity of the leaf was
higher than that of the flower and stem. (C) 2012 Elsevier Ltd. All rights reserved.
Keywords
Polyphenols; Flavonoids; Lonicera japonica Thunb.; HPLC-MS/MS; Antioxidant activity; Metabolomes; Hydroxycinnamic acid
Tags
IRIS
•
Methanol (Non-Cancer)
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WOS
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