US EPA

1452045 

Journal Article 

Identification and Quantification of Phytochemical Composition and Anti-inflammatory and Radical Scavenging Properties of Methanolic Extracts of Chinese Propolis 

Shi, H; Yang, H; Zhang, X; Yu, L( 

2012 

Yes 

Journal of Agricultural and Food Chemistry
ISSN: 0021-8561
EISSN: 1520-5118 

60 

50 

12403-12410 

23176258 

10.1021/jf3042775 

WOS:000312464100023 

Fifteen propolis samples collected from different regions
of China were investigated and compared for their phytochemical composition and anti-inflammatory
and radical scavenging properties. Eleven compounds including caffeic, p-coumaric, ferulic,
isoferulic, and 3,4-dimethylcaffeic acids, pinobanksin, chrysin, pinocembrin, galangin,
pinobanksin 3-acetate, and caffeic acid phenylethyl ester were quantified for the 15 propolis
samples using a UHPLC method, whereas 38 compounds were identified by UPLC/QTOE-MS. The 15
propolis samples significantly differed in their total phenolic and total flavonoid contents, as
well as their phytochemical profiles. The methanol extracts of propolis also showed significant
anti-inflammatory effects in LPS-stimulated RAW 264.7 mouse macrophage cells at 10 mu g propolis
extract/mL concentration. Additionally, the propolis samples differed in their DPPH, ABTS cation,
hydroxyl, and peroxide radical scavenging capacities and ferric reducing abilities. The results
from this study may be used to improve the commercial production and consumption of Chinese
propolis products. 

propolis; phenolic; flavonoid; anti-inflammation; radical scavenging; methanolic extract; chemical consituent