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1452167 
Journal Article 
A single extraction and HPLC procedure for simultaneous analysis of phytosterols, tocopherols and lutein in soybeans 
Slavin, M; Yu, L( 
2012 
Yes 
Food Chemistry
ISSN: 0308-8146
EISSN: 1873-7072 
135 
4 
2789-2795 
22980873 
WOS:000309786400096 
A saponification/extraction procedure and high performance liquid chromatography (HPLC) analysis method were developed and validated for simultaneous analysis of phytosterols, tocopherols and lutein (a carotenoid) in soybeans. Separation was achieved on a phenyl column with a ternary, isocratic solvent system of acetonitrile, methanol and water (48:22.5:29.5, v/v/v). Evaporative light scattering detection (ELSD) was used to quantify beta-sitosterol, stigmasterol, campesterol, and alpha-, delta- and gamma-tocopherols, while lutein was quantified with visible light absorption at 450 nm. Peak identification was verified by retention times and spikes with external standards. Standard curves were constructed (R-2 > 0.99) to allow for sample quantification. Recovery of the saponification and extraction was demonstrated via analysis of spiked samples. Also, the accuracy of results of four soybeans using the described saponification and HPLC analytical method was validated against existing methods. This method offers a more efficient alternative to individual methods for quantifying lutein, tocopherols and sterols in soybeans. (c) 2012 Elsevier Ltd. All rights reserved. 
Soy; Phytosterol; Tocopherol; Lutein; ELSD HPLC; Phenyl column 
IRIS
• Methanol (Non-Cancer)
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