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1452585 
Journal Article 
Evaluation of cytotoxic activity of patriscabratine, tetracosane and various flavonoids isolated from the Bangladeshi medicinal plant Acrostichum aureum 
Uddin, SJ; Grice, D; Tiralongo, E 
2012 
1 
Pharmaceutical Biology
ISSN: 1388-0209 
50 
10 
1276-1280 
English 
22906240 
WOS:000308526600011 
Context: Acrostichum aureum L. (Pteridaceae), a mangrove
fern, has been used as a Bangladeshi traditional medicine for a variety of diseases including
peptic ulcer. Objective: Isolation and structural elucidation of cytotoxic secondary metabolites
from the methanol extract of the aerial parts of A. aureum. Materials and methods: Compounds were
isolated using HPLC. The compound structures were elucidated by 1D and 2D NMR, MS and other
spectroscopic methods using published data. The compounds were tested for their cytotoxic
activity against healthy and cancer cells using the MTT assay. Active compounds were further
evaluated for apoptosis- and necrosis-inducing potential against gastric cancer cells (AGS) using
the FITC Annexin V apoptosis assay. Results and discussion: Seven known compounds,
patriscabratine, tetracosane and 5 flavonoids (quercetin-3-O-beta-D-glucoside, quercetin-3-O-
beta-D-glucosyl-(6 -> 1)-alpha-L-rhamnoside, quercetin-3-O-alpha-L-rhamnoside, quercetin-3-O-
alpha-L- rhamnosyl-7-O-beta-D-glucoside and kaempferol) were isolated. Patriscabratine was found
moderately cytotoxic against AGS, MDA-MB-231 and MCF-7 cells with IC50 values ranging from 69.8
to 197.3 mu M. Tetracosane showed some cytotoxic activity against AGS, MDA-MB-231, HT-29 and NIH
3T3 cells with IC50 values ranging from 128.7 to > 250 mu M. Patriscabratine and tetracosane
displayed an apoptotic effect (10%) on AGS cells within 24 h which was increased (20%) after 48
h, and was comparable to, if not greater, than the positive control, cycloheximide. Conclusion:
Except for quercetin-3-O-beta-D-glucoside and kaempferol; compounds were isolated for the first
time from this plant and evaluated for their cytotoxic activity. The results highlight the
potential of this plant as a source of bioactive compounds and provide a rationale for its
traditional use in peptic ulcer treatment. 
Pteridaceae; Mangrove fern; cytotoxicity; apoptosis and necrosis 
IRIS
• Methanol (Non-Cancer)
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