US EPA

1453585 

Journal Article 

Choline-based deep eutectic solvents for enzymatic preparation of biodiesel from soybean oil 

Zhao, Hua; Zhang, C; Crittle, TD 

2013 

Yes 

Journal of Molecular Catalysis B: Enzymatic
ISSN: 1381-1177 

85-86 

243-247 

10.1016/j.molcatb.2012.09.003 

WOS:000312231000032 

In this study, we introduced choline-based deep eutectic
solvents (such as choline chloride/glycerol at 1:2 molar ratio) as inexpensive, non-toxic,
biodegradable and lipase-compatible solvents for the enzymatic preparation of biodiesel from
soybean oil. Through the evaluation of different eutectic solvents and different lipases. as well
as the study of reaction parameters (i.e. methanol concentration, Novozym 435 loading and
reaction time), we were able to achieve up to 88% triglyceride conversions in 24 h. The enzyme
could be reused for at least four times without losing much activity. Our results indicate that
new benign eutectic solvents can be used as substitutes of toxic and volatile organic solvents in
the enzymatic production of biodiesel from real triglycerides (such as soybean oil). (C) 2012
Elsevier B.V. All rights reserved. 

Biodiesel; Deep eutectic solvent; Enzymatic transesterification; Ionic liquid