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1453600 
Journal Article 
PREPARATIVE ISOLATION AND PURIFICATION OF ALKALOIDS FROM PICRASMA QUASSIODES (D. DON) BENN. BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY 
Zhao, W; He, J; Zhang, Y; Ito, Y; Su, Qi; Sun, W 
2012 
Yes 
Journal of Liquid Chromatography and Related Technologies
ISSN: 1082-6076
EISSN: 1520-572X 
TAYLOR & FRANCIS INC 
PHILADELPHIA 
35 
11 
1597-1606 
English 
22798720 
WOS:000305566900010 
By using a two-phase solvent system composed of n-hexane ethyl acetate-methanol-water (2: 2: 2: 2, v/v/v/v), a high-speed counter-current chromatography
technique was successfully used for isolation and purification of three alkaloids from Picrasma
quassiodes (D. Don) Benn. for the first time. A total of 22.1 mg of 3-methylcanthin-2,6-dione,
4.9 mg of 4-methoxy-5-hydroxycanthin-6-one and 1.2 mg of 1-methoxycarbonyl-beta-carboline were
obtained from 100 mg of crude extract of Picrasma quassiodes (D. Don) Benn. in less than 5 hr,
with purities of 89.30%, 98.32%, and 98.19%, respectively. The target compounds were identified
by ESI-MS, H-1 NMR, and C-13 NMR. 
alkaloids; high-speed countercurrent chromatography; Picrasma quassiodes; 1-methoxycarbonyl-beta-carboline; 3-methylcanthin-2,6-dione; 4-methoxy-5-hydroxycanthin-6-one 
IRIS
• Methanol (Non-Cancer)
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