US EPA

1454594 

Journal Article 

Cloacibacillus porcorum sp. nov., a mucin-degrading bacterium from the swine intestinal tract and emended description of the genus Cloacibacillus 

Looft, T; Levine, UY; Stanton, TB 

2012 

Yes 

International Journal of Systematic and Evolutionary Microbiology
ISSN: 1466-5026
EISSN: 1466-5034 

English 

23041639 

10.1099/ijs.0.044719-0 

A novel anaerobic, mesophilic, amino-acid-fermenting bacterium, designated strain CL-84T, was isolated from the swine intestinal tract on mucin-based media. The bacterium had curved-rod shape (0.8-1.2 µm x 3.5-5.0 µm), stained Gram-negative, and was non-motile with no evidence of spores. CL-84T produces acetate, propionate, formate and butyrate as the end products of metabolism when grown on serine. Optimum growth occurred at 39°C and pH 6.5. The major cellular fatty acids were iso-C15:0, iso-C15:0 3-OH, iso-C17:0, and C16:0, distinguishing CL-84T from closely related species. The G+C content of CL-84T DNA was 55.1 mol%. 16S rRNA gene sequence analysis showed that CL-84T had sequence similarity (90-95%) with characterized genera within the Phylum Synergistetes, Family Synergistaceae. Phylogenetic analysis showed that CL-84T was related to, but distinct from Cloacibacillus evryensis. Based on these findings, we propose that strain CL-84T represents a new species of the genus Cloacibacillus. We further propose the name Cloacibacillus porcorum be designated for this species. CL-84T is the type strain and has been deposited in both DSM and CCUG under accession numbers 25858 and 62631 respectively.