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HERO ID
1467209
Reference Type
Journal Article
Title
Enhanced tolerance to naphthalene and enhanced rhizoremediation performance for Pseudomonas putida KT2440 via the NAH7 catabolic plasmid
Author(s)
Fernández, M; Niqui-Arroyo, JL; Conde, S; Ramos, JL; Duque, E
Year
2012
Is Peer Reviewed?
Yes
Journal
Applied and Environmental Microbiology
ISSN:
0099-2240
EISSN:
1098-5336
Volume
78
Issue
15
Page Numbers
5104-5110
Language
English
PMID
22582075
DOI
10.1128/AEM.00619-12
Web of Science Id
WOS:000306532500009
URL
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84866172978&doi=10.1128%2fAEM.00619-12&partnerID=40&md5=67063ad6a57a3d5ba0d8c7818c069419
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Abstract
In this work, we explore the potential use of the Pseudomonas putida KT2440 strain for bioremediation of naphthalene-polluted soils. Pseudomonas putida strain KT2440 thrives in naphthalene-saturated medium, establishing a complex response that activates genes coding for extrusion pumps and cellular damage repair enzymes, as well as genes involved in the oxidative stress response. The transfer of the NAH7 plasmid enables naphthalene degradation by P. putida KT2440 while alleviating the cellular stress brought about by this toxic compound, without affecting key functions necessary for survival and colonization of the rhizosphere. Pseudomonas putida KT2440(NAH7) efficiently expresses the Nah catabolic pathway in vitro and in situ, leading to the complete mineralization of [(14)C]naphthalene, measured as the evolution of (14)CO(2), while the rate of mineralization was at least 2-fold higher in the rhizosphere than in bulk soil.
Keywords
Medical Sciences; Bioremediation; Gram-negative bacteria; Plasmids; Polycyclic aromatic hydrocarbons; Gene expression; Biodegradation
Tags
IRIS
•
Naphthalene
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Naphthalene (2021 Evidence mapping publication)
Previous HERO references
Database Searches
PubMed
WOS
Combined data set
Data set for title/abstract screening
Excluded – PECO criteria not met
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