US EPA

1467209 

Journal Article 

Enhanced tolerance to naphthalene and enhanced rhizoremediation performance for Pseudomonas putida KT2440 via the NAH7 catabolic plasmid 

Fernández, M; Niqui-Arroyo, JL; Conde, S; Ramos, JL; Duque, E 

2012 

Yes 

Applied and Environmental Microbiology
ISSN: 0099-2240
EISSN: 1098-5336 

78 

15 

5104-5110 

English 

22582075 

10.1128/AEM.00619-12 

WOS:000306532500009 

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84866172978&doi=10.1128%2fAEM.00619-12&partnerID=40&md5=67063ad6a57a3d5ba0d8c7818c069419
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In this work, we explore the potential use of the Pseudomonas putida KT2440 strain for bioremediation of naphthalene-polluted soils. Pseudomonas putida strain KT2440 thrives in naphthalene-saturated medium, establishing a complex response that activates genes coding for extrusion pumps and cellular damage repair enzymes, as well as genes involved in the oxidative stress response. The transfer of the NAH7 plasmid enables naphthalene degradation by P. putida KT2440 while alleviating the cellular stress brought about by this toxic compound, without affecting key functions necessary for survival and colonization of the rhizosphere. Pseudomonas putida KT2440(NAH7) efficiently expresses the Nah catabolic pathway in vitro and in situ, leading to the complete mineralization of [(14)C]naphthalene, measured as the evolution of (14)CO(2), while the rate of mineralization was at least 2-fold higher in the rhizosphere than in bulk soil. 

Medical Sciences; Bioremediation; Gram-negative bacteria; Plasmids; Polycyclic aromatic hydrocarbons; Gene expression; Biodegradation