Investigation of substance P transport across the blood-brain barrier | Health & Environmental Research Online (HERO)

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HERO ID

1469122

Reference Type

Journal Article

Title

Investigation of substance P transport across the blood-brain barrier

Author(s)

Freed, AL; Audus, KL; Lunte, SM

Year

2002

Is Peer Reviewed?

Journal

Peptides
ISSN: 0196-9781

Volume

Issue

Page Numbers

157-165

Language

English

PMID

11814631

DOI

10.1016/S0196-9781(01)00592-7

Web of Science Id

WOS:000173678100021

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036152889&doi=10.1016%2fS0196-9781%2801%2900592-7&partnerID=40&md5=3015b0ab10ede5a0bbb75220de610ea1
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Abstract

The transport of substance P (SP) was investigated using the bovine brain microvessel endothelial cell culture model of the blood-brain barrier (BBB). The samples were derivatized precolumn with naphthalene dialdehyde, then analyzed by cyclodextrin-modified micellar electrokinetic chromatography with laser-induced fluorescence detection. SP crossed the BBB in both the apical-to-basolateral and basolateral-to-apical directions through an active transport mechanism. The transport of SP from the apical side was demonstrated to be via transcytosis. The N-terminal (SP(1-4)) and C-terminal (SP(3-11)) fragments were also found to permeate the BBB from the apical side.

Keywords

substance P; transport; blood-brain barrier; brain microvessel endothelial cells; capillary electrophoresis; laser induced fluorescence detection

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