Separation of naphthalene-2,3-dicarboxaldehyde-derivatized-substance P and its metabolites by micellar electrokinetic chromatography | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

1469280

Reference Type

Journal Article

Title

Separation of naphthalene-2,3-dicarboxaldehyde-derivatized-substance P and its metabolites by micellar electrokinetic chromatography

Author(s)

Freed, AL; Lunte, SM

Year

2000

Is Peer Reviewed?

Yes

Journal

Electrophoresis
ISSN: 0173-0835
EISSN: 1522-2683

Volume

Issue

Page Numbers

1992-1996

Language

English

PMID

10879958

DOI

10.1002/1522-2683(20000601)21:10<1992::AID-ELPS1992>3.0.CO;2-E

Web of Science Id

WOS:000087767800007

URL

http://://WOS:000087767800007
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Abstract

This paper describes the development of analytical methodology for the separation of naphthalene-2,3-dicarboxaldehyde (NDA)-derivatized substance P (CBI-SP) and five lysine-containing metabolites by micellar electrokinetic chromatography (MEKC). The effect of surfactant composition and organic modifiers on the separation was investigated. The final separation buffer consisted of 80 mM sodium cholate in 50 mM N-tris (hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES), pH 7. All six lysine-containing peptides were separated under these conditions.

Keywords

substance P; naphthalene-2,3-dicarboxaldehyde; micellar electrokinetic chromatography; sodium cholate