US EPA

1489499 

Journal Article 

Biotransformation and genotoxicity responses to PAHs in two teleost species 

Maria, VL; Gravato, C; Correia, AC; Santos, MA 

2002 

1 

Fresenius Environmental Bulletin
ISSN: 1018-4619
EISSN: 1610-2304 

11 

9A 

609-615 

English 

WOS:000179431600015 

http://www.psp-parlar.de/details_artikel.asp?tabelle=FEBArtikel&artikel_id=130&jahr=2002
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Eels (Anguilla anguilla L.) were exposed for 8 hours to 2.7 mM naphthalene (Naph), 7-isopropyl-1-methyl-phenanthrene (retene), benzo(a)pyrene (B(a)P), and b-naphthoflavone (BNF), whereas sea bass (Dicentrarchus labrax L.) was exposed for the same duration to the same molar concentration of BNF. Liver biotransformation responses were measured as cytochrome P-450 (P450), 7-ethoxy-resorufin- O-deethylase (EROD) activity and liver EROD/P450 coefficient. The genotoxic effects were determined as erythrocytic micronuclei (EMN test), erythrocytic nuclear abnormalities (ENA test) and DNA strand-breaks from either hepatocytes or blood cells. Eels exhibited a significant liver EROD activity as well as liver EROD/P450 coefficient after 8 hour exposure to 2.7 mM Naph, retene and BNF, whereas the other biomarkers studied were not significantly different when compared to their controls. Eels exposed to B(a)P exhibited an increased liver EROD/P450 coefficient. Liver EROD activity, P450 content, liver DNA strand-breaks, EMN and ENA were significantly increased in sea bass after 8 hours exposure to BNF, whereas liver EROD/P450 was similar to the control. The sea bass results seem to indicate an association between liver biotransformation activity increase, liver DNA integrity decrease as well as EMN and ENA increase. However, liver EROD increase was not associated with those genotoxic effects in eels, probably due to an efficient phase II conjugation and/ or DNA repair mechanism. Therefore, the results show different responses depending on fish species. 

Dicentrarchus labrax; Anguilla anguilla; PAHs; EROD; P450; micronuclei; nuclear abnormalities; DNA-strand-breaks 

11th International Symposium on Environmental Pollution and Its Impact on Life in the Mediterranean Region 

LIMASSOL, CYPRUS