US EPA

1491068 

Journal Article 

ORIGIN AND DEVELOPMENT OF CALLUS FORMATION FROM CULTURED BUD OF VANDA ORCHID 

Kanchanapoom, K; Vajrabhaya, T; Thaitong, O 

1991 

1 

Journal of the Science Society of Thailand
ISSN: 1513-1874 

17 

3-4 

95-102 

WOS:A1991KR65400003 

http://://WOS:A1991KR65400003
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Lateral buds of Vanda x Miss Joaquim were cultured on a
modified agar medium containing 10% coconut water and 0.1 mg/l alpha-naphthalene acetic add for
one month before being transferred to a liquid medium. Histological analysis was carried out on
paraffin sections stained with hematoxylin. The cultured buds on an agar medium resembled a
typical angiosperm shoot apex. After leaf excision, some of these buds initiated meristematic
cells on the abaxial position of young leaves. Serial sections revealed that callus formation
originated from a single meristematic cell that had dense cytoplasm with a large nucleus. The
calli were composed of parenchyma cells bounded by an epidermis-like layer and subsequently these
cells differentiated into protocorm-like bodies when they were submerged in a liquid medium.