US EPA

1496426 

Journal Article 

Ferrocenyl naphthalene diimide can bind to DNA-RNA hetero duplex: potential use in an electrochemical detection of mRNA expression 

Sato, S; Fujii, S; Yamashita, K; Takagi, M; Kondo, H; Takenaka, S 

2001 

Yes 

Journal of Organometallic Chemistry
ISSN: 0022-328X
EISSN: 1872-8561 

637 

476-483 

English 

10.1016/s0022-328x(01)00951-2 

WOS:000172577800061 

http://://WOS:000172577800061
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The novel ferrocenyl naphthalene diimide derivative I was synthesized by the condensation reaction between ferroceneacetic acid and terminal amino moieties of two imide substituents of naphthalene diimide. Stopped-flow analysis of the ligand I and DNA interaction revealed the following results: (i) the ligand binds to double stranded DNA more strongly than to single stranded DNA; (ii) the ligand does not have a bias against DNA sequence; (iii) the ligand can bind to the DNA-RNA hetero duplex more strongly than to the DNA-DNA duplex. These properties of I make it a suitable ligand for analysis of not only DNA but RNA. In fact, a current due to I was observed at 269 mV in the differential pulse voltammetric analysis of dA(20)-immobilized electrode in an electrolyte containing 1. The current increased markedly upon hybridization with polyU, whereas no current increase was obtained for polyA. Since the DNA-RNA hetero duplex is formed between sample RNA and DNA on the electrode, I may be used for the electrochemical detection of mRNA expression. (C) 2001 Elsevier Science B.V. All rights reserved. 

ferrocenyl naphthalene diimide; DNA(.)RNA hetero duplex; Electrochemical detection