US EPA

2306535 

Journal Article 

Overexpression of L-glutamine : D-fructose-6-phosphate amidotransferase provides resistance to methylmercury in Saccharomyces cerevisiae 

Miura, N; Kaneko, S; Hosoya, S; Furuchi, T; Miura, K; Kuge, S; Naganuma, A 

1999 

Yes 

FEBS Letters
ISSN: 0014-5793
EISSN: 1873-3468 

BIOSIS/99/26219 

458 

2 

215-218 

English 

10481068 

10.1016/S0014-5793(99)01158-8 

WOS:000082769200028 

http://www.sciencedirect.com/science/article/pii/S0014579399011588
Exit
 

BIOSIS COPYRIGHT: BIOL ABS. To identify novel genes that confer resistance to methylmercury (MeHg), a yeast genomic DNA library was transfected into Saccharomyces cerevisiae. Two functional plasmids were isolated from transfected yeast clones D1 and H5 that exhibited resistance to MeHg. The yeast transfected with plasmid isolated from clone H5 was several-fold more resistant than yeast transfected with plasmid from clone D1. Functional characterization of the genomic DNA fragment obtained from clone H5 determined that the in did not show resistance to mercuric chloride, zinc chloride, cadmium chloride or copper chloride, suggesting that the resistance acquired by GFA1 gene transfection might be specific to MeHg. This is the first report of a gene involved in MeHg resistance in eukaryotic cells identified by screening a DNA library. 

methylmercury; cytotoxicity; yeast; screening; resistance gene; L-glutamine : D-fructose-6-phosphate amidotransferase