US EPA

2711722 

Journal Article 

Callus induction and plant regeneration from mature seeds of Salvia splendens 

Liu, H; Zhang, GP; Shen, GZ; Ruan, SL; Fu, QJ 

2012 

Yes 

International Journal of Agriculture and Biology
EISSN: 1560-8530 

14 

3 

445-449 

English 

WOS:000304196400021 

The effect of various combinations of plant growth regulators (PGRs) and explant types on the callus induction and plant regeneration of Salvia splendens Ker-Gawl was investigated. Results showed that the medium containing 6-benzylaminopurin (BAP), naphthalene acetic acid (NAA) and 2,4-Dichlorophenoxy acetic acid (2,4-D), could induce callus from stem and leaf explants; whereas, the medium containing only BAP and NAA failed to induce organogenic callus from mature seeds. For the two cultivars examined, Queen and vista, the highest rate of callus induction from leaf and stem explants occurred in the medium containing 1.0 mg L-1 2,4-D and 1.5 mg L-1 BAP; whereas the highest rate of callus induction from mature seeds occurred in the medium containing 1.0 mg L-1 BAP and 2.0 or 2.5 mg L-1 2,4-D. Although shoots were sporadically initiated at very low frequencies from the calli induced from stem segments with nodes, the calli induced from stem or leaf segments did not form shoots. Many regenerated plantlets were obtained from the cotyledon-derived calli, induced from mature seeds and the medium containing 2.0 mg L-1 1-phenyl-3-(1,2,3-thia-diazol-5-YL) urea (TDZ) and 1.0 mg L-1 kinetin(KT), showed the highest efficiency in shoot regeneration of the two S. splendens cultivars. (C) 2012 Friends Science Publishers 

Mature seed; Callus; Regeneration; Plant growth regulator