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Citation
Tags
HERO ID
3857460
Reference Type
Journal Article
Title
Genomic and proteomic characterization of Gordonia sp. NB4-1Y in relation to 6 : 2 fluorotelomer sulfonate biodegradation
Author(s)
Van Hamme, JD; Bottos, EM; Bilbey, NJ; Brewer, SE
Year
2013
Is Peer Reviewed?
1
Journal
Microbiology (Reading, England)
ISSN:
1350-0872
EISSN:
1465-2080
Publisher
MICROBIOLOGY SOC
Location
LONDON
Volume
159
Issue
Pt. 8
Page Numbers
1618-1628
Language
English
PMID
23744905
DOI
10.1099/mic.0.068932-0
Web of Science Id
WOS:000330261900009
Abstract
Gordonia sp. strain NB4-1Y was isolated from vermicompost using bis-(3-pentafluorophenylpropyl)-sulfide as the sole added sulfur source and was found to have a broad capacity for metabolizing organosulfur compounds. NB4-1Y is closely related to G. desulfuricans and was found to metabolize 6 : 2 fluorotelomer sulfonate (6 : 2 FTS) to 5 : 3 fluorotelomer acid (5 : 3 acid) via 6 : 2 fluorotelomer acid (6 : 2 FTCA), 6 : 2 unsaturated fluorotelomer acid (6 : 2 FTUCA) and 5 : 3 unsaturated fluorotelomer acid (5 : 3 Uacid). Given that the molecular and biochemical basis for the microbial metabolism of poly- and per-fluorinated compounds has yet to be examined, we undertook to investigate 6 : 2 FTS metabolism in NB4-1Y. To this end, a whole-genome shotgun sequence was prepared and two-dimensional differential in-gel electrophoresis was used to compare proteomes of MgSO4- and 6 : 2 FTS-grown cells. Of the three putative alkanesulfonate monooxygenases, four nitrilotriacetate monooxygenases and one taurine dioxygenase located in the draft genome, two nitrilotriacetate monooxygenases were differentially expressed in the presence of 6 : 2 FTS. It is hypothesized that these two enzymes may be responsible for 6 : 2 FTS desulfonation. In addition, a differentially expressed putative double bond reductase may be involved in the reduction of 5 : 3 Uacid to 5 : 3 acid. Other proteins differentially expressed during 6 : 2 FTS metabolism included a sulfate ABC transporter ATP-binding protein and two alkyl hydroperoxide reductases. This work establishes a foundation for future studies on the molecular biology and biochemistry of poly- and per-fluorinated compound metabolism in bacteria.
Tags
PFAS
•
5:3 acid
Literature Search
Pubmed
WOS
Screening Results
Excluded/Not on Topic
•
Additional PFAS (formerly XAgency)
Literature Search November 2019
Web of Science
2H-Perfluoro-2-octenoic acid
•
Expanded PFAS SEM (formerly PFAS 430)
Litsearch: September 2019
Web of Science
Not prioritized for screening
6:2 Fluorotelomer sulfonic acid
2H-Perfluoro-2-octenoic acid (6:2)
2-(Perfluorohexyl)ethanoic acid
•
^Per- and Polyfluoroalkyl Substances (PFAS)
FtS 6:2 (27619-97-2)
Literature Search
Pubmed
WOS
5:3 acid (914637-49-3)
Literature Search
Pubmed
WOS
•
PFAS 150
Literature Search Update December 2020
PubMed
WOS
Literature Search August 2019
PubMed
Web of Science
2H,2H,3H,3H-Perfluorooctanoic acid
6:2 Fluorotelomer sulfonic acid
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