US EPA

3858721 

Journal Article 

Perfluoro-N-decanoic acid effects on enzymes of fatty acid metabolism 

Singer, SS; Andersen, ME; George, ME 

1990 

1 

Toxicology Letters
ISSN: 0378-4274
EISSN: 1879-3169 

54 

1 

39-46 

English 

2244339 

10.1016/0378-4274(90)90053-O 

WOS:A1990EJ85800005 

In vitro perfluorodecanoate (PFDA) effects on Pseudomonas acyl-CoA synthetase, Candida acyl-CoA oxidase and pigeon muscle carnitine acetyltransferase were examined. Synthetase made little PFDA-CoA from PFDA. It used palmitate, oleate, laurate and decanoate more extensively. PFDA inhibited acyl-CoA formation from these acids. Palmitoyl-CoA formation was affected most. That of decanoyl-CoA was affected least. Inhibitions appeared to be competitive. Acyl-CoA oxidase test substrates were palmitoyl-CoA, lauroyl-CoA and decanoyl-CoA. Oxidase preferred C-10 and C-12 acyl-CoAs. PFDA inhibited oxidation of C-10 and C-12 acyl-CoAs more than that of palmitoyl-CoA. Inhibitions with C-16 and C-10 acyl-CoAs were competitive, KIs 593 +/- 150 and 76 +/- 6.0 microM. Acetyl-CoA was the best acetyltransferase substrate. C-2 to C-8 transfer from acyl-CoAs was inhibited similarly by PFDA. Inhibitions of C-2 and C-8 transfer were competitive and non-competitive, respectively, KIs 111 +/- 15 and 76 +/- 28 microM. 

Acyl-CoA oxidase; Acyl-CoA synthetase; Carnitine acetyltransferase; Enzymology; Lipid metabolism; Perfluorodecanoic acid