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Citation
Tags
HERO ID
3860276
Reference Type
Journal Article
Title
Perfluorooctane sulfonamide: A structurally novel uncoupler of oxidative phosphorylation
Author(s)
Schnellmann, RG; Manning, RO
Year
1990
Is Peer Reviewed?
1
Journal
Biochimica et Biophysica Acta
ISSN:
0006-3002
EISSN:
1878-2434
Volume
1016
Issue
3
Page Numbers
344-348
Language
English
PMID
2331477
DOI
10.1016/0005-2728(90)90167-3
Web of Science Id
WOS:A1990DC21800005
Abstract
The effects of sulfluramide (N-ethylperfluorooctane sulfonamide) and perfluorooctane sulfonamide (DESFA) on isolated rabbit renal cortical mitochondria (RCM) were examined. Sulfluramid (1-100 microM) and DESFA (0.5-50 microM) increased state 4 respiration of RCM respiring on pyruvate/malate or succinate in a concentration dependent manner in the absence of a phosphate acceptor. In addition, both sulfluramid and DESFA increased state 4 respiration in the presence of oligomycin, an inhibitor of F0F1-ATPase. The effects of sulfluramid (200 microM), DESFA (100 microM), and the known protonophore and uncoupler of oxidative phosphorylation, carbonyl cyanide p-trifluoromethoxyphenylhydrazone (FCCP) (1 microM), on RCM proton movement were examined directly by monitoring extramitochondrial pH and indirectly by monitoring passive mitochondrial swelling. Immediately upon addition, DESFA and FCCP, but not sulfluramid, dissipated the RCM proton gradient and caused RCM to swell in solutions of NaCl or NH4Cl. These results show that DESFA uncouples oxidative phosphorylation by acting as a protonophore. RCM were shown to metabolize sulfluramid to DESFA which suggests that the increase in state 4 respiration observed with sulfluramid is due to DESFA. DESFA is unique in that it is one of two uncouplers that does not contain a ring structure and thus may be a useful model in the study of oxidative phosphorylation.
Tags
PFAS
•
Additional PFAS (formerly XAgency)
•
Expanded PFAS SEM (formerly PFAS 430)
Litsearch: September 2019
PubMed
Not prioritized for screening
Perfluorooctane
•
^Per- and Polyfluoroalkyl Substances (PFAS)
PFOSA (754-91-6)
Literature Search
Pubmed
WOS
•
PFAS 150
Literature Search Update December 2020
PubMed
Literature Search August 2019
PubMed
Screened Studies
Supplemental
N-Ethylperfluorooctanesulfonamide
Perfluorooctane
Perfluorooctanesulfonamide
Perfluorooctanesulfonamide sodium salt (1:1)
•
PFOSA
Literature Search
Pubmed
WOS
Screening Results
In vitro/ex vivo/in silico
Other mechanistic studies
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