US EPA

3860593 

Journal Article 

Heparin micropatterning onto fouling-release perfluoropolyether-based polymers via photobiotin activation 

Credi, C; De Marco, C; Molena, E; Pla Roca, M; Samitier Martí, J; Marques, J; Fernàndez-Busquets, X; Levi, M; Turri, S 

2016 

Yes 

Colloids and Surfaces B: Biointerfaces
ISSN: 0927-7765
EISSN: 1873-4367 

ELSEVIER SCIENCE BV 

AMSTERDAM 

146 

250-259 

English 

27351136 

10.1016/j.colsurfb.2016.06.020 

WOS:000382269600029 

A simple method for constructing versatile ordered biotin/avidin arrays on UV-curable perfluoropolyethers (PFPEs) is presented. The goal is the realization of a versatile platform where any biotinylated biological ligands can be further linked to the underlying biotin/avidin array. To this end, microcontact arrayer and microcontact printing technologies were developed for photobiotin direct printing on PFPEs. As attested by fluorescence images, we demonstrate that this photoactive form of biotin is capable of grafting onto PFPEs surfaces during irradiation. Bioaffinity conjugation of the biotin/avidin system was subsequently exploited for further self-assembly avidin family proteins onto photobiotin arrays. The excellent fouling release PFPEs surface properties enable performing avidin assembly step simply by arrays incubation without PFPEs surface passivation or chemical modification to avoid unspecific biomolecule adsorption. Finally, as a proof of principle biotinylated heparin was successfully grafted onto photobiotin/avidin arrays. 

Polymers; Micropatterning; Antifouling; Perfluoropolyether; Photobiotin; Soft lithography; Microcontact printing; Microcontact arrayer; Heparin; Malaria