Identification and characterization of Gluconacetobacter diazotrophicus mutants defective in the solubilization of phosphorus and zinc | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

4490354

Reference Type

Journal Article

Title

Identification and characterization of Gluconacetobacter diazotrophicus mutants defective in the solubilization of phosphorus and zinc

Author(s)

Intorne, AC; de Oliveira, MV; Lima, ML; da Silva, JF; Olivares, FL; de Souza Filho, GA

Year

2009

Is Peer Reviewed?

Yes

Journal

Archives of Microbiology
ISSN: 0302-8933
EISSN: 1432-072X

Location

Germany

Volume

191

Issue

Page Numbers

477-483

Language

English

PMID

19340412

DOI

10.1007/s00203-009-0472-0

Web of Science Id

WOS:000265497800011

Abstract

Gluconacetobacter diazotrophicus is a plant-growth-promoting bacterium, which is able to colonize sugarcane and other plant species of economic importance. The potentially beneficial effects promoted by this bacterium on plants are nitrogen-fixation, production of phythormones, action against pathogens and mineral nutrient solubilization. In this study, the molecular mechanisms associated with phosphorus and zinc solubilization were analyzed. A transposon mutant library was constructed and screened to select for mutants defective for phosphorous [Ca(5)(PO(4))(3)OH] and zinc (ZnO) solubilization. A total of five mutants were identified in each screen. Both screenings, performed independently, allowed to select the same mutants. The interrupted gene in each mutant was identified by sequencing and the results demonstrate that the production of gluconic acid is a required pathway for solubilization of such nutrients in G. diazotrophicus.

Keywords

Index Medicus