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Citation
Tags
HERO ID
5018500
Reference Type
Journal Article
Title
MS-5, a Naphthalene Derivative, Induces the Apoptosis of an Ovarian Cancer Cell CAOV-3 by Interfering with the Reactive Oxygen Species Generation
Author(s)
Ma, E; Jeong, SJ; Choi, JS; Nguyen, TH; Jeong, CH; Joo, SH
Year
2019
Volume
27
Issue
1
Page Numbers
48-53
Language
English
PMID
29605989
DOI
10.4062/biomolther.2018.020
Web of Science Id
WOS:000455986100006
URL
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067066813&doi=10.4062%2fbiomolther.2018.020&partnerID=40&md5=c8b10f23e94735892ce64bc21fcf11f9
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Abstract
Reactive oxygen species (ROS) are widely generated in biological processes such as normal metabolism and response to xenobiotic exposure. While ROS can be beneficial or harmful to cells and tissues, generation of ROS by diverse anti-cancer drugs or phytochemicals plays an important role in the induction of apoptosis. We recently identified a derivative of naphthalene, MS-5, that induces apoptosis of an ovarian cell, CAOV-3. Interestingly, MS-5 induced apoptosis by down-regulating the ROS. Cell viability was evaluated by water-soluble tetrazolium salt (WST-1) assay. Apoptosis was evaluated by flow cytometry analysis. Intracellular ROS (H₂O₂), mitochondrial superoxide, mitochondrial membrane potential (MMP) and effect on cycle were determined by flow cytometry. Protein expression was assessed by western blotting. The level of ATP was measured using ATP Colorimetric/Fluorometric Assay kit. MS-5 inhibited growth of ovarian cancer cell lines, CAOV-3, in a concentration- and time-dependent manner. MS-5 also induced G1 cell cycle arrest in CAOV-3 cells, while MS-5 decreased intracellular ROS generation. In addition, cells treated with MS-5 showed the decrease in MMP and ATP production. In this study, we found that treatment with MS-5 in CAOV-3 cells induced apoptosis but decreased ROS level. We suspect that MS-5 might interfere with the minimum requirements of ROS for survival. These perturbations appear to be concentration-dependent, suggesting that MS-5 may induce apoptosis by interfering with ROS generation. We propose that MS-5 may be a potent therapeutic agent for inducing apoptosis in ovarian cancer cell through regulation of ROS.
Tags
IRIS
•
Naphthalene
Database Searches
PubMed
WOS
Combined data set
Data set for title/abstract screening
Excluded - PECO criteria not met (TIAB)
Supplemental material
Mechanistic
Mechanisms of cancer
Feb 2019 Update
PubMed
WOS
Other
•
Naphthalene (2021 Evidence mapping publication)
Database Searches
PubMed
WOS
Combined data set
Data set for title/abstract screening
Excluded – PECO criteria not met
Supplemental material
Mechanistic
Mechanisms of cancer
Feb 2019 Update
PubMed
WOS
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