Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
5115044
Reference Type
Journal Article
Title
Design of a simple and novel photoelectrochemical aptasensor for detection of 3,3',4,4'-tetrachlorobiphenyl
Author(s)
Fan, L; Zhang, C; Shi, H; Zhao, G
Year
2019
Is Peer Reviewed?
Yes
Journal
Biosensors and Bioelectronics
ISSN:
0956-5663
EISSN:
1873-4235
Volume
124-125
Page Numbers
8-14
Language
English
PMID
30339976
DOI
10.1016/j.bios.2018.09.054
Abstract
In view of the urgent need of determining polychlorinated biphenyls in the environment, we developed a highly sensitive and selective photoelectrochemical (PEC) aptasensor for determination of 3,3',4,4'-tetrachlorobiphenyl (PCB77) by immobilizing aptamer on N-doped TiO2 nanotubes (N-doped TiO2 NTs). To improve analytical performance of the PEC sensor, the complementary DNA functionalized CdS quantum dots (DNA-CdS QDs) were introduced onto N-doped TiO2 NTs by hybridization. In addition of PCB77, owing to high affinity of aptamer to PCB77, PCB77-aptamer complexes were formed by being bound of PCB77 whilst DNA-CdS QDs were released from the sensing surface. The complexes with poor conductivity hindered the interfacial electron transfer, leading to the photocurrent decrease. The more important is the release of DNA-CdS QDs enhanced the photocurrent decrease, playing the role of signal amplification. The photocurrent change was utilized to detect PCB77 quantitatively. The PEC aptasensor exhibited excellent analytical performance for detection of PCB77 with wide linear range of 0.1-100 ng/L and a low detection limit of 0.1 ng/L. It manifested outstanding selectivity for PCB77 in control experiments by employing six interferents which had similar structure or coexisted with PCB77. Besides, the PEC aptasensor was used to detect the content of PBC77 in the environment.
Tags
IRIS
•
PCBs
Not prioritized for screening
Litsearches
Litsearch: Aug 2018 - Aug 2019
PubMed
Not prioritized for screening
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity