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596365 
Journal Article 
Ozone-induced paradoxical sleep decrease is related to diminished acetylcholine levels in the medial preoptic area in rats 
Alfaro-Rodríguez, A; González-Piña, R 
2005 
Yes 
Chemico-Biological Interactions
ISSN: 0009-2797
EISSN: 1872-7786 
151 
3 
151-158 
English 
15733536 
WOS:000227602400001 
Ozone (O3) produces significant effects on sleep, characterized specially by a decrease in paradoxical sleep (PS) and increase in slow-wave sleep (SWS), which in turn represent a sleep-wake cycle disruption. On the other hand, neuronal activity recorded in the cholinoceptive hypothalamic medial preoptic area (MPO) has been involved in the regulation of sleep. However, there is no direct evidence on the role that acetylcholine (Ach) release in the MPO plays in the sleep-wake cycle. In order to study this relation, we measured the Ach concentration in dialysates collected from MPO in rats exposed to coal-filtered air (clean air) for 48 h and in rats exposed to clean air for 24 h followed by 24-h of O3 exposure to 0.5 ppm. Polygraphic sleep records were taken simultaneously to neurochemical sampling. O3 was employed to disrupt the sleep-wake cycle and relate these changes with concomitant disruptions in Ach concentration dialyzed from MPO. A clear circadian pattern of Ach concentration was observed in dialysates from MPO and also in PS, SWS and wakefulness of rats exposed to filtered air. However, O3 exposure decreased the PS by 65% (Mann-Whitney's U-test, p
Acetylcholine/*metabolism; Air; Animals; Chromatography, High Pressure Liquid; Darkness; Light; Male; Microdialysis; Ozone/*pharmacology; Preoptic Area/drug effects/*physiology; Rats; Rats, Wistar; Sleep, REM/drug effects/*physiology 
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