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646665 
Journal Article 
Separation of phospholipid classes by hydrophilic interaction chromatography detected by electrospray ionization mass spectrometry 
Schwalbe-Herrmann, M; Willmann, J; Leibfritz, D 
2010 
Yes 
Journal of Chromatography A
ISSN: 0021-9673
EISSN: 1873-3778 
1217 
32 
5179-5183 
English 
20598701 
WOS:000280497300006 
A new isocratic separation method was developed for separation of phospholipid (PL) classes based on a silica hydrophilic interaction liquid chromatography (HILIC) column with electrospray ionization (ESI) mass spectrometric detection. Although HILIC is typically used for polar compounds, also amphiphilic molecules like phospholipids can be separated very well. Compared to normal-phase (NP) chromatography, which is usually used for PL class separation, HILIC has the advantage to use on-line ESI-MS detection because its eluents are ESI compatible. Furthermore, this HILIC method is isocratic and hence less time consuming than most (gradient) NP HPLC methods. A chromatographic baseline separation of a standard mixture containing phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), sphingomyelin (SM) and lysophosphatidylcholine (LPC) was achieved within a total run time of 17min using a mobile phase consisting of acetonitrile, methanol and ammonium acetate 10mM. The new method was subsequently tested on phospholipid fractions of a body fluid (human blood plasma) and a tissue extract (swine brain) whereby it achieved nearly the same baseline separation of the PL classes. The detected classes in both cases were PE, PC, SM and LPC. 
Phospholipids; Hydrophilic interaction chromatography (HILIC); Mass spectrometry 
• Methanol (Non-Cancer)
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