Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
730076
Reference Type
Journal Article
Title
Assessment of the cytotoxicity and genotoxicity of haloacetic acids using microplate-based cytotoxicity test and CHO/HGPRT gene mutation assay
Author(s)
Zhang, SH; Miao, DY; Liu, AL; Zhang, L; Wei, W; Xie, H; Lu, WQ
Year
2010
Is Peer Reviewed?
1
Journal
Mutation Research: Genetic Toxicology and Environmental Mutagenesis
ISSN:
1383-5718
EISSN:
1879-3592
Volume
703
Issue
2
Page Numbers
174-179
Language
English
PMID
20801231
DOI
10.1016/j.mrgentox.2010.08.014
Web of Science Id
WOS:000285865400014
Abstract
Haloacetic acids (HAAs) are the second most prevalent class of disinfection byproducts found in drinking water. The implications of HAAs presence in drinking water are a public health concern due to their potential mutagenic and carcinogenic effects. In the present study, we examined the cytotoxic and genotoxic effects of six common HAAs using a microplate-based cytotoxicity test and a hypoxanthine-guanine phosphoribosyltransferase (HGPRT) gene mutation assay in Chinese hamster ovary K1 (CHO-K1) cells. We found that their chronic cytotoxicities (72h exposure) to CHO-K1 cells varied, and we ranked their levels of toxicity in the following descending order: iodoacetic acid (IA)>bromoacetic acid (BA)>dibromoacetic acid (DBA)>chloroacetic acid (CA)>dichloroacetic acid (DCA)>trichloroacetic acid (TCA). The toxicity of IA is 1040-fold of that of TCA. All HAAs except TCA were shown to be mutagenic to CHO-K1 cells in the HGPRT gene mutation assay. The mutagenic potency was compared and ranked as follows: IA>DBA>BA>CA>DCA>TCA. There was a statistically significant correlation between cytotoxicity and mutagenicity of the HAAs in CHO-K1 cells. The microplate-based cytotoxicity assay and HGPRT gene mutation assay were suitable methods to monitor the cytotoxicity and genotoxicity of HAAs, particularly for comparing the toxic intensities quantitatively.
Keywords
Cytotoxicity; Genotoxicity; Haloacetic acids; HGPRT assay; CHO-K1 cells
Tags
•
Trichloroacetic acid (TCA) (Final, 2011)
•
Trichloroethylene (TCE) (Final, 2011)
All References
Hazard
Genotox
OPPT REs
•
OPPT_Trichloroethylene (TCE)_F. Human Health
Total – title/abstract screening
On topic
Peer review
Primary source
Cited in IRIS document or IRIS HERO page
On topic - additional tags for titles/abstracts
MOA
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity