Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
8361395
Reference Type
Journal Article
Title
Characterization of unsubstituted and methylated polycyclic aromatic hydrocarbons in settled dust: Combination of instrumental analysis and in vitro reporter gene assays and implications for cancer risk assessment
Author(s)
Hoang, AQ; Suzuki, G; Michinaka, C; Tue, NM; Tuyen, LH; Tu, MB; Takahashi, S
Year
2021
Is Peer Reviewed?
1
Journal
Science of the Total Environment
ISSN:
0048-9697
EISSN:
1879-1026
Publisher
Elsevier B.V.
Location
AMSTERDAM
Volume
788
Page Numbers
147821
Language
English
PMID
34029822
DOI
10.1016/j.scitotenv.2021.147821
Web of Science Id
WOS:000662646300011
URL
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85107029379&doi=10.1016%2fj.scitotenv.2021.147821&partnerID=40&md5=c789a22ff91198642d3a294a0a8968eb
Exit
Abstract
Concentrations of 34 unsubstituted and methylated polycyclic aromatic hydrocarbons (PAHs and Me-PAHs) and AhR-mediated activities in settled dust samples were determined by a combination of gas chromatographyâmass spectrometry and an in vitro reporter gene assay (PAH-CALUX). The levels of Σ34PAHs and bioassay-derived benzo[a]pyrene equivalents (CALUX BaP-EQs) were significantly higher in workplace dust from informal end-of-life vehicle dismantling workshops than in common house dust and road dust. In all the samples, the theoretical BaP-EQs of PAHs (calculated using PAH-CALUX relative potencies) accounted for 28 ± 19% of the CALUX BaP-EQs, suggesting significant contribution of aryl hydrocarbon receptor (AhR) agonists and/or mixture effects. Interestingly, the bioassay-derived BaP-EQs in these samples were significantly correlated with not only unsubstituted PAHs with known carcinogenic potencies but also many Me-PAHs, which should be included in future monitoring and toxicity tests. The bioassay responses of many sample extracts were substantially reduced but not suppressed with sulfuric acid treatment, indicating contribution of persistent AhR agonists. Cancer risk assessment based on the CALUX BaP-EQs has revealed unacceptable level of risk in many cases. The application of bioassay-derived BaP-EQs may reduce underestimation in environmental management and risk evaluation regarding PAHs and their derivatives (notably Me-PAHs), suggesting a consideration of using in vitro toxic activity instead of conventional chemical-specific approach in such assessment practices. © 2021 Elsevier B.V.
Keywords
Methylated PAHs; PAH-CALUX; PAH-induced toxicity; PAHs; Settled dust
Tags
IRIS
•
PCBs
Excluded
Litsearches
Litsearch: Aug 2020 - Aug 2021
WoS
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity