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HERO ID
990585
Reference Type
Journal Article
Title
Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)
Author(s)
Chen, GY; Qiu, SL; Zhou, YY
Year
2009
Is Peer Reviewed?
1
Journal
Research in Microbiology
ISSN:
0923-2508
EISSN:
1769-7123
Volume
160
Issue
3
Page Numbers
173-178
Language
English
PMID
19640417
DOI
10.1016/j.resmic.2009.01.003
Web of Science Id
WOS:000267685500001
URL
http://linkinghub.elsevier.com/retrieve/pii/S0923250809000047
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Abstract
Classical cultivation and molecular methods based on the ammonia monooxygenase gene (amoA) were used to study the abundance and diversity of beta-proteobacterial ammonia-oxidizing bacteria (AOB) in lake sediments. The eutrophic and oligotrophic basins of a Chinese shallow lake (Lake Donghu), in terms of ammonium (NH(4)(+)) concentrations, were sampled. The AOB number was significantly lower in the oligotrophic basin, but significantly higher in the eutrophic basin. In addition, using restriction fragment length polymorphism targeting the amoA, ten restriction patterns including six unique ones were found in the eutrophic basin, while five patterns were observed in the oligotrophic basin with only one unique restriction group. Phylogenetic analysis for AOB revealed that Nitrosomonas oligotropha- and Nitrosomonas ureae-related AOB and Nitrosospira-affiliated AOB were ubiquitous; the former dominated in the eutrophic basin (87.2%), while, the latter dominated in the oligotrophic basin (65.5%). Furthermore, Nitrosomonas communis-related AOB was only detected in the eutrophic basin, at a small proportion (3.2%). These results indicate significant selection and adaptation of sediment AOB in lakes with differing trophic status.
Keywords
Ammonia-oxidizing bacteria; Ammonia monooxygenase gene; Restriction fragment length polymorphism; Shallow lakes
Tags
IRIS
•
Ammonia
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