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993294 
Journal Article 
Effects of methionine and lysine on fermentation in vitro and in vivo, nutrient flow to the intestine, and milk production 
Chung, YH; Bateman, HG; Williams, CC; Stanley, CC; Gantt, DT; Braud, TW; Southern, LL; Ward, JD; Hoyt, PG; Sod, GA 
2006 
Yes 
Journal of Dairy Science
ISSN: 0022-0302
EISSN: 1525-3198 
89 
5 
1613-1620 
English 
16606731 
Three experiments were conducted to investigate the feasibility of using crystalline methionine and lysine as protein supplements for lactating Holstein cows. In the first experiment, Met (dl-methionine) and Lys (l-lysine-HCl) were added to diets used in continuous culture bioreactors to estimate optimal concentrations for use in subsequent in vivo experiments. The second experiment measured ruminal fermentation and nutrient flow to the small intestine when Met and Lys were top-dressed on diets fed to nonlactating cows. The third experiment measured lactation performance when Met and Lys were added to diets fed to late-lactation cows. Providing 0.29 and 2.27% of dry matter as Met and Lys, respectively, provided the largest improvement in fermentation in vitro and these concentrations were used in subsequent experiments. When Met and Lys were top-dressed on diets fed to nonlactating cows, no changes in total tract N digestion were observed. No changes in microbial protein production or ruminal fermentation were observed. Adding Met and Lys did not change production or efficiency of production of milk or milk components by late lactation cows. These data indicate that providing supplemental Met and Lys during late lactation does not significantly improve the protein status of the cow and therefore may not improve milk production.