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Citation
Tags
HERO ID
1029178
Reference Type
Journal Article
Title
Comparative biochemical and transcriptional profiling of two contrasting varieties of Brassica juncea L. in response to arsenic exposure reveals mechanisms of stress perception and tolerance
Author(s)
Srivastava, S; Srivastava, AK; Suprasanna, P; D'Souza, SF
Year
2009
Is Peer Reviewed?
Yes
Journal
Journal of Experimental Botany
ISSN:
0022-0957
EISSN:
1460-2431
Volume
60
Issue
12
Page Numbers
3419-3431
Language
English
PMID
19528528
DOI
10.1093/jxb/erp181
Web of Science Id
WOS:000269014600011
Abstract
The mechanisms of perception of arsenic (As)-induced stress and ensuing tolerance in plants remain unresolved. To obtain an insight into these mechanisms, biochemical and transcriptional profiling of two contrasting genotypes of Brassica juncea was performed. After screening 14 varieties for As tolerance, one tolerant (TPM-1) and one sensitive (TM-4) variety were selected and exposed to arsenate [As(V)] and arsenite [As(III)] for 7 d and 15 d for biochemical analyses. The tolerant variety (TPM-1) demonstrated higher accumulation of As upon exposure to both 500 microM As(V) and 250 microM As(III) [49 microg g(-1) and 37 microg g(-1) dry weight (dw) after 15 d] as well as a better response of thiol metabolism as compared with the responses observed in the sensitive variety (TM-4). Transcriptional profiling of selected genes that are known to be responsive to sulphur depletion and/or metal(loid) stress was conducted in 15-d-old seedlings after 3 h and 6 h exposure to 250 microM As(III). The results showed an up-regulation of sulphate transporters and auxin and jasmonate biosynthesis pathway genes, whereas there was a down-regulation of ethylene biosynthesis and cytokinin-responsive genes in TPM-1 within 6 h of exposure to As(III). This suggested that perception of As-induced stress was presumably mediated through an integrated modulation in hormonal functioning that led to both short- and long-term adaptations to combat the stress. Such a coordinated response of hormones was not seen in the sensitive variety. In conclusion, an early perception of As-induced stress followed by coordinated responses of various pathways was responsible for As tolerance in TPM-1.
Keywords
Arsenic; Brassica juncea; hormone regulation; real-time PCR; stress perception; thiol metabolism
Tags
IRIS
•
Arsenic Hazard ID
1. Initial Lit Search
PubMed
WOS
ToxNet
4. Considered through Oct 2015
6. Cluster Filter through Oct 2015
•
Arsenic (Inorganic)
1. Literature
PubMed
Toxline, TSCATS, & DART
Web of Science
4. Adverse Outcome Pathways/Networks Screening
Excluded/Not relevant
Title/Abstract screening
•
Arsenic MOA
1. MOA Literature Screening
MOA Cluster
3. Excluded
Other not relevant
Dragon Screened
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