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Tags
HERO ID
1039270
Reference Type
Journal Article
Title
Combined use of isopropylamine and trifluoroacetic acid in methanol-containing mobile phases for chiral supercritical fluid chromatography
Author(s)
De Klerck, K; Mangelings, D; Clicq, D; De Boever, F; Vander Heyden, Y
Year
In Press
Is Peer Reviewed?
Yes
Journal
Journal of Chromatography A
ISSN:
0021-9673
EISSN:
1873-3778
Language
English
PMID
22142971
DOI
10.1016/j.chroma.2011.11.023
Web of Science Id
WOS:000302831100012
Abstract
In chiral supercritical fluid chromatography (SFC), mobile-phase additives are often used to improve enantioseparations and peak shapes. An acidic or basic additive is chosen, depending on the nature of the compound. This work highlights the simultaneous use of the acidic additive trifluoroacetic acid (TFA) and the basic additive isopropylamine (IPA) in supercritical fluid chromatography for enantioseparations. To evaluate the combination of TFA and IPA, 59 chiral pharmaceutical compounds were analyzed on four polysaccharide-based chiral stationary phases (CSPs): Lux(®) Cellulose-1, Lux(®) Cellulose-2, Lux(®) Cellulose-4 and Lux(®) Amylose-2. The results show that an important increase in enantioselectivity of the chromatographic system can occur when combining trifluoroacetic acid and isopropylamine in the mobile phase (MP), compared to the individual use of these additives. However, the combination of isopropylamine and trifluoroacetic acid in a supercritical methanol-containing mobile phase can also lead to problems as a result of the formation of salt complexes between the two additives. Combining the additives trifluoroacetic acid and isopropylamine and taking the appropriate measures to avoid salt formation, i.e. reducing the additives' concentrations, can lead to simpler chiral SFC screening conditions that display even broader enantioselectivity.
Keywords
Isopropylamine; Trifluoroacetic acid; Mobile phase additives; SFC; Methanol-containing mobile phase; Chiral separations
Tags
IRIS
•
Methanol (Non-Cancer)
Search 2012
PubMed
Search Jan 30 2013
WOS
PFAS
•
PFAS 150
Literature Search Update December 2020
PubMed
Literature Search August 2019
PubMed
Not prioritized for screening
Trifluoroacetic acid
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