US EPA

1244037 

Journal Article 

Evidence of antiapoptotic properties of Pleurotus florida lectin against chronic arsenic toxicity in renal cells of rats 

Rana, T; Bera, AK; Bhattacharya, D; Das, S; Pan, D; Das, SK 

2012 

Yes 

Journal of Environmental Pathology, Toxicology and Oncology
ISSN: 0731-8898
EISSN: 2162-6537 

31 

1 

39-48 

English 

22591283 

10.1615/JEnvironPatholToxicolOncol.v31.i1.50 

WOS:000305846400005 

Chronic arsenic exposure results in toxicity in humans and causes many toxicologic manifestations. Apoptosis was measured by cell adhesion, morphologic alterations, cell proliferation, terminal deoxyuridine triphosphate nick-end labeling (TUNEL), and caspase-3/CPP32 fluorometric protease assay. Results of the present study suggested that arsenic administration in rats caused apoptosis by elevating morphologic alterations, TUNEL-positive nuclei, caspase-3 activity, and DNA damage and by reducing cell adhesion and cell proliferation in a time-dependent manner. The apoptosis in renal cells of arsenic-exposed rats reverted to normal values after coadministration of mushroom lectin. This study provided significant evidence that Pleurotus florida lectin has an antiapoptotic property by protecting from arsenic-induced toxicity. The beneficial effect of Pleurotus florida lectin was proportional to its duration of exposure. This finding might be of therapeutic benefit in people suffering from chronic exposure to arsenic from natural sources, a global problem that is especially relevant to millions of people on the Indian subcontinent. 

arsenic; Pleurotus florida lectin; apoptosis; renal cells