US EPA

1445171 

Journal Article 

Seasonal influence on bioactivity of seaweeds against plant pathogenic bacteria Xanthomonas axonopodis pv. citri (Hasse) Vauterin et al 

Arunkumar, K; Sivakumar, SR 

2012 

Yes 

African Journal of Microbiology Research
ISSN: 1996-0808 

6 

20 

4324-4331 

10.5897/AJMR11.977 

WOS:000307405500017 

In the present study, thin-layer chromatography (TLC)
method was adopted for the fractionation of antibacterial substances from the crude extracts
obtained in 1:1(v/v) chloroform : methanol of red seaweeds comprising Portieria hornemannii
(Lyngbye) P. Silva, Gracilaria crassa (Harvey) J. Ag., brown Dictyota bartyrensiana Lamouroux,
Hydroclathrus clathratus (C. Agardh) M. A. Howe, Sargassum wightii Greville, green Ulva
(Enteromorpha) compressa (Linnaeus) Nees, and Halimeda macroloba Decaisne collected along the
Coast of Pamban (Rameswaram, Gulf of Mannar, India) at four different seasons namely post-monsoon
(February), summer (May), pre-monsoon (August) and monsoon (November) seasons for the year 2009.
Fractionated substances detected under UV (Mechanical + disinfection) and iodine were tested for
antibacterial activity against the plant pathogenic bacterium Xanthomonas axonopodis pv. citri
(Hasse) Vauterin et al. which cause canker in citrus. Number of active fractions with high
antibacterial activity was recorded from the crude extracts obtained in seaweeds collected during
monsoon and post-monsoon whereas seaweeds collected during summer and pre-monsoon showed low
antibacterial activity. UV active substances fractionated at R-f 0.23 from red seaweed Porteiria
hornemannii showed maximum antibacterial activity among the fractionated substances obtained on
TLC from the crude extracts of all the seaweeds investigated. 

Seaweeds; antibacterial activity; Xanthomonas axonopodis pv. Citri; thin-layer chromatography (TLC) fractions